Shah DK, Sanap-Tandale A, Aggarwal S, Sengar E, Borkar A, Shetty S. Comparative evaluation of the sealing ability of filling materials on root end cavities treated with smear layer removing agents: A confocal laser scanning microscopic study. J Conserv Dent 2019;22:495-9
Aim: This study aims to evaluate and compare the sealing ability of Biodentine™ and mineral trioxide aggregate (MTA) plus® on root end cavities treated with 17% ethylene diamine tetraacetic acid (EDTA), 0.2% Chitosan and 1% Phytic acid using Confocal Laser Scanning Microscope (CLSM)-An in vitro study.
Materials and Methods: Sixty extracted single rooted teeth were instrumented and obturated with gutta-percha. The apical 3 mm of each tooth was resected and 3 mm root-end preparation was made using ultrasonic tip. 17% EDTA (n = 20), 0.2% Chitosan (n = 20) and 1% Phytic acid (n = 20) was used as a smear layer removing agent and each above group was further subdivided and restored with a root end filling material, Biodentine (n = 10) and MTA Plus (n = 10). The samples were coated with varnish except at the root end and after drying, they were immersed in Rhodamine B dye for 24 h. The teeth were then rinsed, sectioned longitudinally, and observed under CLSM.
Results: In the present study, MTA Plus® treated with 1% Phytic acid showed the least microleakage followed by Biodentine™ treated with 1% Phytic acid which was statistically not significant. MTA Plus® treated with 17% EDTA showed the highest microleakage when compared to other tested groups. There was a significant difference in microleakage between MTA Plus® and Biodentine™ when treated with 17% EDTA and 0.2% Chitosan. However, more microleakage was seen with Biodentine™ group than MTA plus® group.
Conclusion: Root end cavities restored with MTA plus and treated with Phyitc acid showed superior sealing ability. Furthermore, smear layer removing agents will aid in better adaptability of root end filling material.
Clinical Oral Investigations (2020Abstract
To compare the clinical behavior of a universal light-curing, ultra-fine particle hybrid composite and successor of this material in class I and II cavities after 60 months.
Materials and methods
Forty patients (21 females, 19 males) with ages ranging between 18 and 38 years (23.15 ± 5.15) received 80 (13 Cl I and 67 Cl II) resin composite restorations (Charisma/Charisma Classic, Kulzer GmbH) in combination with an etch and rinse adhesive system (Gluma 2Bond) under rubber dam isolation. Two experienced operators performed all the restorations. Restorations were evaluated by the other two examiners according to the FDI criteria at baseline and at 6, 12, 18, 24, 36, 48 and 60 months. Surface characteristics of one restoration selected randomly were examined under a scanning electron microscope (SEM) at each recall. Data were analyzed statistically (p < 0.05).
After 60 months, recall rate was 90%. None of the restorations failed. Three restorations from Charisma and 4 from the Charisma Classic group showed minor surface staining. Twelve Charisma and 14 Charisma Classic restorations were scored as 2 for margin staining. Four restorations from both groups showed minor shade deviations but no significant difference was observed between the two restorative materials for any criteria evaluated after 60 months (p > 0.05). SEM evaluations were in accordance with the clinical findings.
Both materials exhibited clinically similar and successful performance over the 60-month observation period.
A new formulation of resin composite may not always perform better clinical performances.
Nagaraja S, Mathew S, Jain N, Jethani B, Nambiar S, Kumari M, Nair S. Study of antibacterial and antifungal efficacy of platelet-rich fibrin and platelet-rich fibrin matrix. J Conserv Dent [serial online] 2019 [cited 2020 Aug 5];22:415-9. Available from: http://www.jcd.org.in/text.asp?2019/22/5/415/291315
Background: Platelet concentrates are extensively utilized in the medical and dental field to promote tissue regeneration. The profusion of endogenous growth factors in platelets α-granules transmit their use for enhanced wound healing. However, little attention has been given to study their antimicrobial potential. This study was conducted to assess the antibacterial and antifungal property of platelet-rich fibrin (PRF) and PRF matrix (PRFM).
Materials and Methodology: Blood samples were obtained from 16 participants, PRF and PRFM were processed as per the protocol prescribed by Choukroun et al. and Lucarelli et al., respectively. The susceptibility test against microbiota in the root canal and Candida albicans was assessed through minimum inhibition zone by agar diffusion technique.
Results: PRF showed an effective antibacterial property, however, did not perform well against C. albicans strains. PRFM did not show any antibacterial or antifungal properties.
Conclusions: The antibacterial efficacy of PRF may prove beneficial when used in the revascularization procedure of immature necrotic teeth.